An Attempt to Establish an Agrobacterium-mediated Transient Expression in Euphorbia tirucalli (L.) an Important Medicinal Plant

Background: Optimization of co-cultivation parameters during Agrobacterium-mediated transformation to Euphorbia tirucalli evaluated were bacterial density, infection period, acetosyringone (AS) concentration and temperature. Optimized resulted in high efficiency 3 fold increase at transient GUS expression .The optimized conditions Agrobacterium tumefaciens growth phase A600nm 0.17, period 30 min, addition medium (100 µM) cocultivation temperature 25°C. Methods: Nodal explants used for transformation. Bacterial culture was added 50 ml liquid YEP with kanamycin rifampicin grown until reaching the (A600nm). density ranged from 0.56, 0.8 1.2 OD present study. The made solid MS consisted NAA 2 mg L-1 various concentrations AS 0, 50, 100, 200, 400, 600 800 µM. Histochemical analysis gus gene carried out. Result: Higher more efficiency, but also higher necrosis explants. Dilution suspension reduced is 72% when process out µM). Our studies proved that among conditions, critical mediated